Mutagenesis Analysis of ABCB8 Gene Promoter of Danio rerio

The ABCB8 is one of the members under ABCB subfamily ATP-Binding Cassette (ABC) transporter which possess ability in regulating intracellular iron and heme transport. loss function mutation gene leads to accumulation cell highly toxic human. However, information regarding expression regulation this remains scarce. Hence, objectives project are determine transcription factors binding site (TFBS) identify transcriptional roles cis-elements through mutagenesis analysis. To examine this, total genomic DNA was extracted from Danio rerio promoter sequence isolated by using specific pair primers polymerase chain reaction (PCR). sample sent for sequencing result showed 98% similarities zebrafish clone DKEYP-87A6 linkage group 24. Besides, TFBS studied aspect abundance, composition distribution. two most abundant TFBSs based on liver-specific profile were HNF-3β C/EBPβ, with 38 39 sites, respectively. mutated substitution AP-1 at location 535 other nucleotides a mutagenic (forward primer: 5’-TGGGGGTTTAGATATTGAAAC-3’; reverse 5’-AACTCGC ATACATTTCAGTCATC-3’). This may benefit development new diagnostics therapeutics iron-associated disorder.